ImmunAID Postdoctoral Research Fellow Leeds Institute of Rheumatic & Musculoskeletal Medicine

Post Summary: Applications are invited for a Postdoctoral Research Fellow position in the research group of Professor Michael McDermott on a project funded by the Horizion 2020 entitled “Immunome project consortium for Autoimflammatory disorders (ImmunAID)

Project Description:

You will co-ordinate projects related to Work Package 3 (WP3) of ImmunAID programme entitled: “Inflammasome-associated pathways in sporadic and undiagnosed systemic autoinflammatory disorders (SAID)”. The overall goal of the tasks in WP3) is to assess the range of functions, dysfunction and biomarker potential of NLRP3 inflammasome complexes in SAID. Specifically, you will be working on task 3.3 of WP3 with the following aim: To characterise negative regulators of NLRP3 inflammasome activation, including the effects of decoy proteins, encoding CARD-only proteins (COPs) and the related PYRIN domain-only proteins (POPs), that competitively bind to integral inflammasome components.

We are using standard laboratory-based methods to investigate NLRP3 inflammasome activation products and the unfolded protein response (UPR) in cells from patients with SAIDs and selected cell lines. A Seahorse Analyser is used to examine metabolic pathways and quantify mitochondrial ROS (mROS) production. Cryo-electron microscopy (Cryo-EM) and bioimaging is used for structural molecular biology in collaboration with the Astbury Centre (Leeds).

Reporting to Prof McDermott, your work will be carried out in collaboration with Dr. Sinisa Savic, capitalising on the infrastructure and samples collected through the Leeds Centre for SAIDs, based across St James’s University Hospital and Chapel Allerton Hospital. You will have access cells and sera (blood) from patients with genetically defined SAID and also those with sporadic or unknown causes. You will develop and validate assays for use in cell culture supernatants and biological fluids. Some of the work will be done in conjunction with the Protein Production Facility (run by Dr Brian Jackson) and the Centre for Biomolecular Interactions (run by Dr Iain Manfield), both located within the Faculty of Biological Sciences in the Astbury Centre for Structural Molecular Biology.

In combination with one other Postdoctoral Research Fellow, you will support the management of the research group, specifically dedicated to this project, as required.

The post will be based primarily in the Wellcome Trust Brenner Building at the St. James’s University Hospital site within the Institute of Rheumatic and Musculoskeletal Medicine.

Location: Leeds - St James University Hospital

Faculty/Service: Faculty of Medicine & Health

School/Institute: Leeds Institute of Rheumatic & Musculoskeletal Medicine (LIRMM)

Category: Research

Grade: Grade 7

Salary: £33,797 to £40,322 p.a.

Post Type: Full Time

Contract Type: Fixed Term (From start date to 17 March 2023)

Release Date: Wednesday 06 October 2021

Closing Date: Monday 25 October 2021

Reference: MHLRM1168

To Apply: Please go here

Status: Accepting applications.

Deadline: Monday 25 October 2021.

For more details download the Candidate Brief.

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